Hajra Choudhary Workshop Technology Vol 2 Pdf Free Download EXCLUSIVE

Hajra Choudhary Workshop Technology Vol 2 Pdf Free Download EXCLUSIVE





 
 
 
 
 
 
 

Hajra Choudhary Workshop Technology Vol 2 Pdf Free Download

Hajra Choudhary Workshop Technology Pdf Free DownloadThe goals of this research are to develop, optimize, and commercialize a specific and sensitive electrochemical DNA assay, using a nanobeads-based hybridization capture technique to label, detect, and quantify the gene of interest, and to test its efficacy using commercial transgenic canola (Canola, Brassica napus). This assay takes advantage of the unique chemistry of nanobeads that couple biotinylated single stranded DNA (ssDNA) in a highly controlled one-step protocol. In this system, capture of biotinylated ssDNA on streptavidin coated magnetic nanobeads is achieved through biotin-streptavidin interactions, and the ssDNA on the biotinylated nanobeads is then complexed with complementary single stranded DNA (cDNA). Previous work has shown that the encapsulation of ssDNA on nanobeads greatly improves the efficiency of hybridization capture, compared to surface-based hybridization. The goal of this Phase I effort is to develop a highly sensitive and specific electrochemical DNA assay using an ssDNA-coated nanobeads hybridization capture technique. This is a high-value assay, as it allows for genetic analysis of the desired target DNA in a highly-efficient and rapid fashion. Moreover, the fact that this assay does not require sophisticated instrumentation for DNA hybridization could result in a low-cost and easy-to-use assay for both academic and industrial researchers. In this Phase I application, an innovative electrochemical detection method has been developed to meet the detection sensitivity requirements of the assay while allowing an ultralow detection limit. The novel electrochemical detection method used in the assay is based on the unique redox property of nanobeads, which transforms the nanobeads from an insulator (off state) to a conductor (on state) when hybridized to the complementary DNA. Hence, the on-to-off transition of nanobeads can be easily detected through the direct manipulation of the nanobead’s redox properties, providing a sensitive and reliable method for the electrochemical detection of the genetic sequence.The action of the regulatory neurotransmitter serotonin (5-hydroxytryptamine, 5-HT) at the 5-HT(1A) receptor has been implicated in the pathogenesis of several serious brain disorders such as Alzheimer’s disease, depression, and eating disorders, and it has been hypothesized that the 5-

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